All the tools you need to analyse your miRNAs

DeAnnIso


Target Prediction Sequencing Analysis Target Functional Analysis Differential Expression Analysis IsomiRs Identification


Analysis Scheme:
DeAnnIso, is an online tool, that is designed for Detection and Annotation of IsomiR from small RNA sequencing data. The detected isomiRs will be classified into different categories. The isomiRs will be aligned with canonical miRNA, and will be annotated with expression, constitution, SNP, and an in-house dataset. It can also extract differentially expressing isomiR between two samples. In addition, IsomiR Bank provides targets prediction and enrichment analysis to evaluate the effects of isomiRs on target selection.

Homepage: Link

Last Software Update: May 13, 2016
Publication Date: May 13, 2016
Citations: 8 [via Pubmed]

Organism Specific:
  • human
  • mouse
  • fish
  • fly
  • plants (selectable)

  • Reference Genome Needed:
    Online/Local: /
    Installation/User Level: easy
    User Adjustability:
    User support:
    Precomputed Target Results Available For Download:
    Cross-Refferences:
  • NCBI
  • AmiGO2

  • Target Prediction
    Algorithm Features:
  • seed match
  • conservation
  • free energy
  • target-site abundance
  • 3' compensatory pairing
  • G:U pairs allowed in the seed

  • Target Region: 3'UTR, CDS
    Target Annotation:
    Algorithm: miRanda, RNAhybrid
    NGS Data Needed:
    Metaserver:
    Sequencing Analysis
    Algorithm Features:
  • differential expression
  • target prediction
  • target annotation
  • isomiRs identyfication

  • Sequencing Platform Specific: Illumina
    PARE/CLIP-Seq: /
    Differential Expression Analysis
    Algorithm: Wilcoxon Rank-sum test
    miRNA differential expression:
    mRNA differential expression:
    NGS Data Needed:
    Target Functional Analysis
    Algorithm: DAVID
    Analysis Features:
  • GO Terms
  • KEGG Pathways
  • NGS Data Needed:
    Target Prediction Data Needed:
    IsomiRs Identification
    NGS Data Needed:





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